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	Provedor de dados Acta Botanica (1) Genet. Mol. Biol. (1) Autor Adriano-Anaya,Lourdes (1) Han,Ying Ying (1) Ming,Feng (1) Ovando-Medina,Isidro (1) Salvador-Figueroa,Miguel (1) Sánchez-Gutiérrez,Adriana (1) Vázquez-Ovando,Alfredo (1) Yan,Qin Hua (1) Mais... Palavra-chave MiR172 (2) Flowering (1) Homologous cloning (1) Jatropha curcas (1) MiR156 (1) MiRNA (1) MiRNAs (1) Phalaenopsis (1) Sequence conservation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida Genet. Mol. Biol. Han,Ying Ying; Yan,Qin Hua; Ming,Feng. MiR172 is an important microRNA that regulates floral development in various plants and downregulates AP2 family members to relieve the stress on floral determinacy, leading to phase transition from vegetative to reproductive growth. In this work, PCR with primers designed based on the rice miR172 sequence was used to isolate two miR172-like transcripts from Phalaenopsis hybrida (PhmiR172-1 and PhmiR172-2) that were very similar to Oryza miR172d and Arabidopsis miR172b. RT-PCR indicated that the levels of these two transcripts were negatively correlated with the level of the Phalaenopsis AP2 (PhAP2) gene in stem, root, pedicel and sepal, and that both were co-expressed with PhAP2 in young buds. Overproduction of PhmiR172-2 in Arabidopsis led to early... Tipo: Info:eu-repo/semantics/article Palavras-chave: Homologous cloning; MiR172; MiRNA; Phalaenopsis; Sequence conservation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300014 Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L. Acta Botanica Sánchez-Gutiérrez,Adriana; Ovando-Medina,Isidro; Adriano-Anaya,Lourdes; Vázquez-Ovando,Alfredo; Salvador-Figueroa,Miguel. ABSTRACT Jatropha curcas is a plant that accumulates high-quality oil in its seeds, which is capable of being transformed into liquid biofuel. However, several aspects of its flowering are still unknown, which is a key step in the production of fruits, seeds, and oil. Flowering is a complex process that is regulated by various factors at the molecular level, including microRNAs (miRNAs). There is evidence suggesting that the miRNAs of the miR156 and miR172 families play a key role in the flowering transition of plants. For this reason, the dynamics of miR156 and miR172 were studied during a production cycle of J. curcas. Our results reveal that J. curcas has a mechanism for the expression of these miRNAs that differs from that reported for the vast... Tipo: Info:eu-repo/semantics/article Palavras-chave: Flowering; Jatropha curcas; MiR156; MiR172; MiRNAs. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100099 Registros recuperados: 2 Primeira ... 1 ... Última

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